Immunochemical determination of LD-1 and LD-2 in CK-MB fraction of column eluates.
نویسندگان
چکیده
منابع مشابه
Immunochemical determination of CK-MB isoenzyme in human serum. II. An enzymic approach.
A novel immunochemical technique for a specific enzymic determination of the myocardial isoenzyme of creatine kinase, CK-MB, involves determination of B-subunit activity of a specimen in which the M-subunit activity has been inhibited by specific antibodies to the M-subunit. Interfering activities from CK-BB isoenzyme, atypical forms of creatine kinase, and adenylate kinase are eliminated by us...
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The presence of creatine kinase isoenzyme MB (CK-MB) in human serum is an indicator of myocardial injury. In this assay for CK-MB, reagent containing both rabbit antibodies to CK-MM and 125I-labeled sheep antibodies to CK-BB is added to the patient's serum and incubated for 1 h at ambient temperature. Goat anti-rabbit immunoglobulin, conjugated to a mixture of amylose and polyvinylidene fluorid...
متن کاملA two-tube immunochemical method for determination of CK-MB isoenzyme in serum evaluated.
A new commercial kit (Impres-MB; International Immunoassay Labs.) recently was introduced for measuring the MB isoenzyme of creatine kinase (CK-MB) based on the use of monoclonal antibodies. After antibodies to CK-MM isoenzyme are added to precipitate the CK-MM, antibodies to CK-M monomer are added to precipitate the M-subunit isoenzymes of CK. Subtracting the enzymatic activity of the second s...
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Recent advances in analytic techniques have increased the diagnostic value of creatine kinase MB (CK-MB), enabling earlier and more sensitive results. The CK-MB mass immunoassays, that utilise the monoclonal anti-CK-MB in conjunction with anti-M or anti-B antibodies, are able to measure accurately small changes during the early hours after myocardial infarction (MI). CK-MB has two main limitati...
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Effects of acute and chronic desipramine treatment. We compared an immunochemical method for determination of creatine kinase (CK; EC 2.7.3.2) and lactate dehydrogenase (LD; EC 1.1.1.27) activities with the conventional electropho-retic method. Of the 105 patients included in this study, 58 were in the intensive-care unit with a preliminary diagnosis of myocardial infarction; the other 47 had d...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1981
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/27.5.779